Aim
To obtain understanding of and insight into the molecular biology of mammalian cells with emphasis on signal transduction including transcriptional control, and on oncogenes and tumor suppressor genes.
Contents
The theory will concern elements in signal transduction pathways by going through receptors and kinases and effectors as interferons and insulin. The elements in transcriptional control in mammalian cells will concern cis-elements (DNA), RNA polymerase and transcription factors. Finally, oncogenes and tumor suppressor genes will be treated according to their "normal function".
Practical work: Mammalian cells culture; transfection of cells with reporter- and expression plasmids; treatment of transformed cells with effectors as interferons and dexamethasone; reporter gene analysis, western blots, PCR and gel mobility shifts. Computers will be used to collect and treat data.
Form and duration: 4 Lessons/colloquia per week for 6 weeks. Evaluation day: 8 hours. Practial course: 10 days of 6 hours (in August).
Compulsory programme: Symposiums and practical laboratory work.
Necessary qualifications: Molecular Biology A, Biochemistry A and Molecular Cell Biology A.
Comments: Max. 18 participants.
Text-books
Roger J.B.King: "Cancer Biology", 2nd ed., Prentice Hall, 2000.
Original articles. Practical. Course guide.
Evaluation
Take home assignments and an oral evaluation of approx. 20 minutes. Marks according to the 13 scale.
ECTS-credits
10
Semester
Fall