Aim
To use yeast for studies of the molecular biological organization and regulatory processes of eukaryotic cells.
Contents
The issues are primarily dealt with through lectures and symposiums based on original literature. The laboratory course gives a practical demonstration of some of the central biological principles and number of modern techniques. Throughout yeast cells are used as the biological system. Among other things the practical laboratory course will include cloning in E. coli and yeast, investigation of the stability of different types of plasmids in yeast, isolation of genes by complementation in yeast, investigation of the chromosome localization of yeast genes by pulsed field-gelectrophoresis, and integration of genes in the yeast genome by homologous recombination (gene disruption, gene targeting).
Form and duration: 36 hours of symposium/lectures and 50 hours of practical laboratory work.
Compulsory programme: Symposiums and practical laboratory work.
Necessary qualifications: Molecular Biology A.
Text-books
Original articles, laboratory manual and H. Lodish et al. "Molecular Cell Biology", 4.ed., 2000.
Evaluation
7-days term-paper (13-scale).
ECTS-credits
10
Semester
Spring