Molecular Biology of Yeast Cells
Aim To use yeast for studies of the molecular biological organization and regulatory processes of eukaryotic cells.
Contents The issues are primarily dealt with through lectures and symposiums based on original literature. The laboratory course gives a practical demonstration of some of the central
biological principles and number of modern techniques. Throughout yeast cells are used as the biological system. Among other things the practical laboratory course will include
cloning in
E. coli and yeast, investigation of the stability of different types of plasmids in yeast, isolation of genes by complementation in yeast, investigation of the chromosome
localization of yeast genes by
pulsed field-gelectrophoresis, and integration of genes in the yeast genome by homologous recombination (
gene disruption, gene targeting).
Form and duration: 36 hours of symposium/lectures and 50 hours of practical laboratory work.
Compulsory programme: Symposiums and practical laboratory work.
Necessary qualifications: Molecular Biology A.
Text-books Original articles, laboratory manual and H. Lodish
et al. "Molecular Cell Biology", 4.ed., 2000.
Evaluation 7-days term-paper (13-scale with external examiner).
ECTS-credits 10
Semester Spring