Contents
Objective
Genome sequencing programs of model organisms have identified many new genes
of unknown function. The course will give an overview of different experimental
approaches to study the function of these genes.
The course starts with a general introduction to basic techniques in genome analysis
such as mapping as well as cloning- and sequencing strategies. Then we will discuss
some examples of finished genome sequences of model organisms, and which conclusions
one can draw from these. The main focus of the course lies in the description of a
series of experimental design to unravel gene functions, which are based on genome
sequence information, cDNAs and proteins. Applications in biotechnology and medicin
will also be discussed.
Headlines
- Generation and computer analysis of genomic sequences
- Technologies of genome wide expression analysis: SAGE, DNA microchip
- Genome wide mutational analysis: Transposon mutagenesis programs
- Genome wide protein networks, Yeast two-hybrid
- Proteomics using mass spectrometry
Form
Three hours a week for ten weeks. Two hours discussions of original articles is
followed by one hour of lecturing on the following weeks topic. Web based computer
practicals and guest lectures will be integrated.
Evaluation
Participation in the course - (passed not passed).
ETCS-credits
5
Semester
Spring 2000