Aim
The course, which is specially directed towards masters- and PhD-students, will concentrate on the increasing usage of phage display libraries in the characterisation of molecular
interactions, e.g. selection of peptide and antibody libraries. Recent technological improvements allow selection based on structure/stability and for catalysis to be performed. These
novel approaches will be covered in detail.
Contents
The theoretical part will include considerations necessary for the construction of libraries as well as will provide an understanding of selection strategies based on affinity capture,
protein stability and enzymatic catalysis. Other in vitro evolution strategies will be included where appropriate. The practical part provides "hand on" experience in the selection of
phage displayed antibody and peptide libraries, together with characterisation of individual clones.
A mini symposium will be included in the theoretical part, where internationally recognised people in the field will present their work.
Form and duration: 2 hours per week for 8 weeks, 4x4 lab-exercises for one week.
Mandatory programme: All the theoretical and practical parts.
Necessary qualifications: Molecular Biology A, Biochemistry A and Molecular Cell Biology A.
Note: Max 12 participants.
Text-books
Original papers.
Evaluation
Pass/Not pass based on a minimum of 80% attendance and active participation in group discussion.
ECTS-credits
5
Semester
Spring